DETERMINATION OF FREE RADICAL SCAVENGING ACTIVITY OF OROXYLUM INDICUM AND QUERCUS INFECTORIA BY REVERSED-PHASE HPLC.

Abstract

In the present study, free radical scavenging activities of the two plants, Oroxylum indicum (L) Kurz and Quercus infectoria G. Olivier were investigated. Free radical scavenging activities of stem bark of O. indicum and insect galls of Q. infectoria were characterized by the HPLC method developed by D. Chandrashekhar, et al, 2005 using Waters 100 RP-18e column (250 mm × 4 mm, 5 µM) in which stable 1,1-diphenyl-2-picrylhydrazyl (DPPH•) free radical was used. The mobile phase used was a mixture of methanol and water (80: 20, v/v) pumped at a flow rate of 1 ml/min. the DPPH peaks were monitored at 517 nm. In the present investigation it was found that both the plant extracts had remarkable free radical scavenging activities. Ascorbic acid and trolox were used as the standard antioxidants. The IC₅₀ values (inhibition concentration 50) of the ethanolic extracts of stem bark of O. indicum and insect galls of Q. infectoria were found to be 72 ± 2.32 and 58 ± 3.17 µg/ml, respectively as determined by the HPLC method, and correlated well with that determined by colorimetric method, i.e., 71.5 ± 3.64 µg/ml for O. indicum and 61.5 ± 2.42 µg/ml for Q. infectoria. The present study suggested that both the plants have potent antioxidant activity.