SYNTHESIS OF SILVER NANOPARTICLES AND ITS CYTOTOXIC EFFECT AGAINST THP-1 CANCER CELL LINE

Abstract

Nanotechnology is a field of science which involves the synthesis and applications of nanoparticles. Silver nanoparticles (AgNp) have applications in various fields due to their unique optical, physical, electrical and medicinal properties. In this study, silver nanoparticles were synthesized by biophysical method. Silver nanoparticles were characterized by using UV-Visible absorption spectroscopy (Surface Plasmon absorption at 418-420 nm), Energy Dispersive Spectroscopy (EDX), Scanning Electron Microscopy (SEM) and X- ray diffraction (XRD). The cytotoxic effect of silver nanoparticles on acute myeloid leukemia cell line (THP – 1 cell line) was studied in vitro. The activity of chemotherapeutic drugs Cyclophosphamide and Busulfan was enhanced in presence of silver nanoparticles whereas Mercaptopurine was found to be more effective when used individually against the THP- 1 cell line.