HER-2/NEU RECEPTOR GENE ISOLATION STUDY FROM INDONESIAN BREAST CANCER PATIENTS TISSUE

Abstract

Breast cancer is a deadly disease, occupied as first ranking among woman patients cancer in Indonesia (the Hospital Information System, 2007). 20-30% of total breast cancer patients having HER-2/neu overexpression. Unfortunately the problem of resistance to monoclonal antibody treatment, trastuzumab, was found. There are several theories that suspected as the cause of trastuzumab resistence such as polimorphisme, receptor dysfunction and so on. This research was conducted to study and to isolate of the HER-2/neu receptor gene in breast cancer patients Indonesia. The successful of the HER-2/neu receptor gene issolation is an important stage for the study and analysis of HER-2/neu receptor in Indonesia. Research methods were breast cancer tissue collection from Dharmais Cancer Hospital and M. Djamil Hospital Padang, RNA isolation, cDNA, PCR, sequencing. Of the total 110 samples, 20 samples of breast cancer patients identified as HER-2/neu+3 through immunohistochemistry (IHC) screening. Of the 20 samples,4 DNA fragments (2127bp) have successed isolated. This  DNA fragment sequenced, then  identified as a gene encoding HER-2/neu receptor using BLAST. Due to the variation of HER-2/neu overexpression  in the tissue sample, while the part is taken as a source of RNA isolation is only a little of it, the possibility to use of non HER-2/neu overexpression part had a large, and other reason RNA low stability were also affected to the success of a gene encoding HER-2/neu receptor issolation.