HPLC Method for Analysis of Lercanidipine Hydrochloride in Tablets

Abstract

A  reverse  phase  HPLC  method  was  developed  for  quantitative  determination  of Lercanidipine  hydrochloride  in  tablets.  The  separation  was  achieved  by  using  250 Χ 4.6mm  Wakosil C₁₈  (5µm)  column  with  a  mixture  of  methanol: acetonitrile (70:30)  as a mobile phase,  at  a  flow  rate  of  1.0ml/min.  The  detection  was  carried  out  at  219nm,  and the  retention  time  was  found  to  be  4.10min. Linearity  was  observed  in  the  concentration range  of  10-60µg/ml.  The  mean  recoveries  obtained  for  lercanidipine  HCl  ranged  from 96.38  to  101.23 %.  The  LOD  and  LOQ  was  found  to  be  0.03µg/ml  and  0.04µg/ml respectively.  The  developed  method  was  found  to  be  accurate,  precise  and  rapid  for analysis  of  Lercanidipine  HCl  in  tablets.