QUANTIFICATION OF N-ACETYLNEURAMINIC ACID( NEU5AC) IN HUMAN MILK USING N- GLYCOLYLNEURAMINIC ACID AS INTERNAL STANDARD BY LC-MS/MS

Authors

  • D.KARUNANITHI Department of Chemistry, National Institute of Technology, Tiruchirappalli, Tamilnadu - 620 015, India.Shriram Institute for Industrial Research, Bangalore, Karnataka - 560 048, India.
  • A.RADHAKRISHNA Shriram Institute for Industrial Research, Bangalore, Karnataka - 560 048, India.
  • V.M.BIJU Department of Chemistry, National Institute of Technology, Tiruchirappalli, Tamilnadu - 620 015, India.

Keywords:

N-acetylneuraminic acid (Neu5Ac). N-glycolylneuraminic acid (Neu5Gc). LC-MS/MS. Human milk

Abstract

A rapid, reliable and sensitive LC-MS/MS method for the determination of N-acetylneuraminic acid (Neu5Ac) in human milk was developed and validated.  Samples were hydrolysed with mild hydrochloric acid and analysed by LC-MS/MS on Thermo hypersilgold C-18   analytical column. The analytical procedure was found to be more selective, accurate, precise and linear. The method accuracy was 99.9 % and the mean precision (RSD) was 3.51 % and the calibration was linear from 0.1 to 10 µg mL-1 (R2> 0.99), the lowest limit of quantification (LLOQ) was 0.1 µg mL-1   .  N-glycolylneuraminicacid (Neu5Gc) was used as an internal standard. This method was proved as promising method for the determination of Neu5Ac in human milk samples.

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Published

31.12.2012

How to Cite

D.KARUNANITHI, A.RADHAKRISHNA, & V.M.BIJU. (2012). QUANTIFICATION OF N-ACETYLNEURAMINIC ACID( NEU5AC) IN HUMAN MILK USING N- GLYCOLYLNEURAMINIC ACID AS INTERNAL STANDARD BY LC-MS/MS. International Journal of Pharma and Bio Sciences, 3(4), 299–308. Retrieved from https://ijpbs.net/index.php/journal/article/view/1681

Issue

Volume 3, Issue 4, 2012

Section

Research Articles

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