Exploitation Of Fruit Wastes For Pectinase Production Using Aspergillus Oryzae

Abstract

Ever increasing population and industrialization has resulted in heavy pollution and waste generation. The main aim of this project lies in waste management and synthesis of pectinase enzyme which plays a crucial role in sewage treatment. Twenty moulds were isolated from decaying fruits and municipal waste soil sample on SDA and were further screened for pectinolytic enzyme production. Screening, isolation and identification of Aspergillus oryzae was done based on larger clearance zones in pectin - Congo red agar media and pectinase enzyme production was carried out inYEP media by submerged fermentation. Further production media was formulated using various fruit wastes (peels) in different compositions. Growth characteristics of the isolate and kinetics of the Exopectinase enzyme were optimized. Sapota peel in combination with groundnut oil cake (GOC) had been proved to be the best media for production of high potential Exopectinase. A oryzae has its highest enzyme activity at 50⁰ C temperature at 4.5 pH concentration level. It takes 60 mins of time duration to give best enzyme activity in1.5% substrate concentration.