MOLECULAR CHARACTERIZATION OF FRESH WATER ACTINOBACTERIA USING 16S RDNA BASED TECHNIQUE
Keywords:
Fresh water, Actinomycetes, Primers, NCBI, BLAST.Abstract
Twenty four isolates of actinomycetes were collected from water and sediment samples of
three major fresh water systems of Karimnagar, Andhra Pradesh, India at Lower Manair
Dam, Manakondur Pond and Kothapally pond from July 2006 to June 2008. These isolates
were screened for their diversity and biological significance. Among them, eight showed
good antagonistic activity against ten test bacteria. Two of these eight isolates (LAM1 and
LAM2) were found to produce wide spectrum of antibacterial agents. These most potent
strains were selected and were subjected to molecular identification. 16S rDNA was
successfully amplified from the genome of the selected isolates with 8F and 1492R primers
using BDT v3.1 cycle sequencing kit on ABI 3730xl Genetic analyzer. 16S rDNA amplicons
of the strains were subjected to automated DNA sequencing. Partial 16S rDNA sequences
obtained was compared directly with sequences in the NCBI database using BLAST as well
as with the sequences available with Ribosomal Database Project (RDP). The sequence
analysis revealed that LAM1 belongs to Streptomyces sp and LAM2 belongs to
Micromonospora sp.
.