Extraction, Partial Characterization And Antibacterial Efficacy Of Extra Cellular Polysaccharides From Bacillus Licheniformis And Klebsiella Pneumoniae Isolated From Root Nodule Of Tephrosia Purpurea

Abstract

Many bacteria produce extra cellular polysaccharide (EPS) and in genera such as Klebsiella, Rhizobium, Bacillus and Streptococcus they[Delete] have been well characterized for a number of species and strains. Polysaccharides from plant sources material and their modified forms are important additives in the food industry. The EPS is the use of xanthan in oil industry. The most significant biomedical exploitation of bacterial polysaccharides is used as vaccine agent. In the present study, the authors describe the selection of the plant Tephrosia purpurea was selected[delete], since it contains many species producing EPS. From the root nodule of this plant, five different species of bacteria were isolated and studied for their antibacterial activity. Out of five strains, two strains showed promising activity against human pathogens (Escherichia coli, Enterococcus aeruginosa, Klebsiella pneumoniae, Staphylococcus aureus) and these two strains were used for further studies. Molecular characterization for best strains was done and found to be Bacillus licheniformis and Klebsiella pneumonia. The growth curves of B.licheniformis and K. pneumoniae were studied. Also EPS were isolated and estimated by phenol sulphuric acid method in UV-Vis spectrophotometer. The large amounts of EPS 210 mg/ml from K.pneumoniae and 70.02 mg/ml from B.licheniformis were estimated before dialysis and after dialysis 12.3 mg/ml from K.pneumoniae and 10.97 mg/ml from B.licheniformis were estimated. Three polysaccharides components were observed by HPLC analysis. Also lyophilization was being used for the production of extra cellular polysaccharides.