Estimation Of Granisetron Hydrochloride By A New Rp-Hplc Method

Authors

  • T.NAGESWARA RAO Department of Analytical Chemistry, International Inisitituate of Biotechnology and Toxicology (IIBAT), Padappai, Kancheepuram, 601301, Tamil Nadu.
  • D.SREENIVASULU Orchid chemicals& pharmaceuticals Ltd, Chennai.
  • P.U.V. SATISH Department of Analytical Chemistry, International Inisitituate of Biotechnology and Toxicology (IIBAT), Padappai, Kancheepuram, 601301, Tamil Nadu.
  • T.PARVATHAMMA Department of Analytical Chemistry, International Inisitituate of Biotechnology and Toxicology (IIBAT), Padappai, Kancheepuram, 601301, Tamil Nadu.

Keywords:

Granisetron hydrochloride, RP-HPLC method, method validation

Abstract

A simple, sensitive and specific reverse phase high performance liquid chromatographic method has been developed for the determination of granisetron hydrochloride in injectable dosage forms. Chromatographic separation was achieved on a peerless basic C18 (250×4.6 mm), 5.0 µm column with a diluted 1.6 ml of ortho phosphoric acid in 800 ml water, added 200ml acetonitrile, added 1.0ml of Hexylamine and mixed. Then adjusted the pH to 7.5 with triethylamine as mobile phase, detection was at 304 nm. Response was a linear function of concentration in the range 5-0.02 mg/L for granisetron hydrochloride. LOD and LOQ for granisetron hydrochloride were found 0.01 mg/L and 0.03 mg/L. Accuracy (recoveries 94-97%) and reproducibility were found to satisfactory.

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Published

30.09.2012

How to Cite

T.NAGESWARA RAO, D.SREENIVASULU, P.U.V. SATISH, & T.PARVATHAMMA. (2012). Estimation Of Granisetron Hydrochloride By A New Rp-Hplc Method. International Journal of Pharma and Bio Sciences, 3(3), 412–419. Retrieved from https://ijpbs.net/index.php/journal/article/view/1500

Issue

Volume 3, Issue 3, 2012

Section

Research Articles

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