Extraction Of Pectinase From Pectinolytic Bacteria Isolated From Carrot Waste

Authors

  • R. C. PATIL Department of Microbiology, Bhavan’s college, Andheri (West), Mumbai- 400058, Maharashtra, India
  • TUSHAR P. MURUGKAR Department of Microbiology, Bhavan’s college, Andheri (West), Mumbai- 400058, Maharashtra, India
  • SHAMIM A. SHAIKH Department of Biochemical Sciences Rajiv Gandhi Institute for IT and Biotechnology, Katraj, Bharati Vidyapeeth University, Pune -411043, Maharashtra, India

Keywords:

Pectinase, vegetable waste, carrot waste, Enzyme kinetics, Pectinolytic bacteria

Abstract

Degradation of waste especially that of vegetable waste has become a mounting challenge owing to its generation in huge amounts. This vegetable market generated waste can be utilized to biosynthesize pectinase enzyme at cheaper rate which have numerous industrial applications. Screening was carried out to obtain pectinase producing microorganisms from carrot. The best isolate was identified as a unique strain of Bacillus sp. based on morphological, biochemical tests and using 16S rRNA gene sequencing studies. This isolate was selected for further bulk production using the optimized conditions of pH 9 and temperature of 50oC to give a maximum yield of 49.58% from carrot waste.

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Published

31.03.2012

How to Cite

R. C. PATIL, TUSHAR P. MURUGKAR, & SHAMIM A. SHAIKH. (2012). Extraction Of Pectinase From Pectinolytic Bacteria Isolated From Carrot Waste. International Journal of Pharma and Bio Sciences, 3(1), 261–266. Retrieved from https://ijpbs.net/index.php/journal/article/view/1252

Issue

Volume 3, Issue 1, 2012

Section

Research Articles

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