Purification Of Epidermal Growth Factor By Displacement Chromatography

Authors

  • RAMESH BHAGAM Department of Biotechnology , Gland Pharma Limited, Hyderabad, A.P, India
  • K.NAGAIAH Department of Biotechnology , Gland Pharma Limited, Hyderabad, A.P, India
  • M. LAXMI NARASU Director-IST, JNTU, Hyderabad, A.P, India
  • A.NARESH Department of Biotechnology , Gland Pharma Limited, Hyderabad, A.P, India

Keywords:

Biosimilars, Epidermal Growth Factor, Displacement chromatography, Methyltrialkyl (C8-C10) ammonium chloride, Benzyldimethyltetradecylammonium chloride (BDTA), HPLC.Displacement Chromatography, BDTA, Adogen 464, Biosimilars, Epidermal Growth Factor.

Abstract

A method was developed based on displacement chromatography technique using inertsil ODS-3V, 4.6 x 250 mm, 5µ HPLC column for the purification of recombinant human Epidermal Growth factor. Care was taken starting from selection of suitable displace, displacer concentration, flow rate etc.We found that Methyltrialkyl (C8-C10) ammonium chloride (Adogen-464) was found to be suitable for separation of epidermal growth factor in purification process. This method was effective in achieving a purity of greater than 99 % with the recovery of 60% which had not been obtained in other chromatographic techniques and this method was proven to be ideal and cost effective.

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Published

31.03.2012

How to Cite

RAMESH BHAGAM, K.NAGAIAH, M. LAXMI NARASU, & A.NARESH. (2012). Purification Of Epidermal Growth Factor By Displacement Chromatography. International Journal of Pharma and Bio Sciences, 3(1), 38–45. Retrieved from https://ijpbs.net/index.php/journal/article/view/1227

Issue

Volume 3, Issue 1, 2012

Section

Research Articles

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