Phenolic Quantification And Antioxidant Activity Of Anaxagorea Dolichocarpa And Duguetia Chrysocarpa (Annonaceae)

Abstract

This work presents the phenolic quantification and evaluation of in vitro antioxidant activity of A. dolichocarpa and D. chrysocarpa are the two species of the Annonaceae family. The total phenolics content of the plant extracts was determined by the Folin-Ciocalteu method. Antioxidant activities were evaluated by using DPPH radical scavenging and β-carotene-linoleic acid bleaching and compared with ascorbic acid, BHA and BHT used as reference compounds. The most significant total phenolic content was of 252.50 ± 7.02 mg of gallic acid equivalent/g for CHCl₃ extract from A. dolichocarpa, which presented the best antioxidant activity (IC₅₀ 55.64 ± 5.24 µg/ml) for DPPH scavenging. For D. chrysocarpa, the more significant total phenolic content was of 213.11 ± 9.87 for AcOEt extract, with IC₅₀ value of 26.97 ± 1.90 µg/ml for DPPH scavenging. BHT was the most effective antioxidant. The results obtained show that phenolic compounds contribute to the antioxidant activity of the extracts. Further studies will be conducted to isolate the chemical constituents responsible for antioxidant activity.