10.22376/ijpbs.2019.10.1.p1-12 Volume 1 Issue 4 2010 (October - December) A novel method for the purification of recombinant protein 24 (rp24) of human immunodeficiency virus type 1 (HIV- 1) from Escherichia coli is described. The protein was over-expressed in E. coli (BL21 lDE3) cells to the levels of ~ 30 % total cell protein in the soluble fraction. The recombinant protein was extracted and purified to near homogeneity by ion-exchange steps followed by ultrafiltration. The recombinant protein in combination with synthetic peptides from immuno-dominant stretches derived from the 'envelope' proteins of HIV-1 and HIV-2 was used for the development of an ELISA kit, for detection of antibodies against the virus in human serum and/or plasma.  S. C. Singh,M. Vasudevan,Richa,R. P. Sinha ELISA, rp24, synthetic peptides, ultrafiltration 287-294