International Journal of Pharma and Bio Sciences
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10.22376/ijpbs.2019.10.1.p1-12
Volume 2 Issue 3
2011 (July - September)
Determination Of Auxotrophic Nature Of The mutant Micrococcus Glutamicus Ab100
An experimental study was carried out to investigate the auxotrophic nature of the newly developed mutant lessThan i greaterThan Micrococcus glutamicus lessThan /i greaterThan AB lessThan sub greaterThan 100 lessThan /sub greaterThan . To investigate the role of biotin on growth and l-glutamic acid production by this strain, yeast extract from the minimal salt medium was replaced by 0.2 µg/ml of biotin. Biotin (0.2 µg/ml) was proved to be an essential element for growth production was decreased significantly (p lessThan 0.01). But excess biotin created over growth, leading to anaerobic condition which could adversely affect l-glutamic acid production. Thus, in this present study, it was proved that this strain a biotin auxotroph.
S. Ganguly And A. K. Banik
Experimental, Micrococcus glutamicus, biotin, auxotroph.
60-63