10.22376/ijpbs.2019.10.1.p1-12 Volume 2 Issue 2 2011 (April - June) Genetic characterization of two species of fruit flies belonging to the genus lessThan i greaterThan Bactrocera lessThan /i greaterThan (= lessThan i greaterThan Dacus lessThan /i greaterThan ), namely, lessThan i greaterThan B. lessThan /i greaterThan lessThan i greaterThan cucurbitae lessThan /i greaterThan and lessThan i greaterThan B. dorsalis lessThan /i greaterThan was carried out using ten RAPD markers. In lessThan i greaterThan B. lessThan /i greaterThan lessThan i greaterThan cucurbitae lessThan /i greaterThan and lessThan i greaterThan B. dorsalis lessThan /i greaterThan twenty four and twenty one fragments were amplified ranging from 150bp-2000bp, respectively. Four out of ten primers were monomorphic. Mean heterozygosity in lessThan i greaterThan B. cucurbitae lessThan /i greaterThan and lessThan i greaterThan B. dorsalis lessThan /i greaterThan was 0.119 and 0.093, respectively. Nei's genetic identity value (0.831), calculated using Tools For Population Genetic Analysis (TFPGA) software, reveals very close genetic similarity between the two species. R. Singh,N. Bajpai And R. R. Tewari RAPD-PCR, Bactrocera, molecular markers, heterozygosity, genetic identity. 498-503