Optimization Of Physical Conditions For The Production Of L-Glutamic Acid By A Mutant Micrococcus Glutamicus AB100.

10.22376/ijpbs.2019.10.1.p1-12

Volume 2 Issue 2 2011 (April - June) Optimization Of Physical Conditions For The Production Of L-Glutamic Acid By A Mutant Micrococcus Glutamicus AB100. Experimental studies were carried out to improve L-glutamic acid production using a biotin requiring auxotrophic mutant lessThan i greaterThan Micrococcus glutamicus lessThan /i greaterThan AB100 by optimizing different physical parameters namely initial pH, period of incubation, volume of medium, age of inoculum, volume of inoculum and temperature. Maximum production of L-glutamic acid was obtained with initial pH, 6.5; period of incubation, 72h; volume of medium, 20ml; age of inoculum, 48h; volume of inoculum, 4% and temperature, 29 lessThan sup greaterThan o lessThan /sup greaterThan C. Production was increased significantly (p lessThan 0.01) from 6.8 to 10.4 mg/ml. Dry cell weight changed significantly (p lessThan 0.01) from 2.3 to 4.6 mg/ml. S. Ganguly And A. K. Banik L-glutamic acid, physical parameters, incubation, medium, inoculum 295-299