10.22376/ijpbs.2019.10.1.p1-12 Volume 2 Issue 2 2011 (April - June) Thermophilic lipase producing bacteria have been isolated and characterized from an Indian hot spring, isolates named as AK-P1, AK-P2 and AK-P3 respectively. The strain AK-P2 was first of its kind with reference to its lipase producing potential. The phylogenetic analysis of these strains using 16S rDNA sequence data revealed that the strain AK-P1 had highest homology (100%) with lessThan i greaterThan Acinetobacter lessThan /i greaterThan sp. 01B0;, AK-P2 had highest homology (99.9%) with lessThan i greaterThan Brevibacillus borstelensis lessThan /i greaterThan ; AR9 and AK-P3 have shown 99.2 % similarities with lessThan i greaterThan Porphyrobacter cryptus lessThan /i greaterThan (T); ALC-2. Maximal lipase production by AK-P2 was observed at the end of the stationary phase (36 h). The optimum temperature and pH for this crude enzyme activity was 60ºC and 10.0 respectively. Enzyme extracted from AK-P2 exhibited a Km 0.052mM and Vmax 0.645 μM/min/ml with p-nitrophenyl laurate as a substrate. Bisht Satpal Singh And Amrita Kumari Panda Lipases, thermophiles, hot spring, 16S rDNA 229-235