10.22376/ijpbs.2019.10.1.p1-12 Volume 2 Issue 2 2011 (April - June) Since ancient time, lessThan i greaterThan Phyllunthus niruri lessThan /i greaterThan L. are using in many herbal preparations. Increasing demand of lessThan i greaterThan P. niruri lessThan /i greaterThan lead to adulteration, and thus there is a global need to develop quality plant material. In present investigation, an efficient lessThan i greaterThan in vitro lessThan /i greaterThan technology was developed for a rapid and large-scale production of lessThan i greaterThan P. niruri lessThan /i greaterThan via axillary bud proliferation using nodal segments of the mature plants. Elongated shoots were cultured on half strength MS medium fortified with various auxins such as NAA, 2, 4-D, IBA, IAA and at different concentrations (0.5 - 5.0 mg/l). lessThan i greaterThan In vitro lessThan /i greaterThan developed plantlets were harden and acclimatized in natural environment. The antimicrobial activity (by well diffusion method) and antioxidant activity (by DPPH scavenging assay, reducing power and total antioxidant capacity methods) of lessThan i greaterThan in vitro lessThan /i greaterThan developed plant, naturally grown plant and commercial available sample of lessThan i greaterThan P.niruri lessThan /i greaterThan were determined. Results of antimicrobial activity of methanol extract from lessThan i greaterThan in vitro lessThan /i greaterThan cultured lessThan i greaterThan P.niruri lessThan /i greaterThan showed great potential as source of antimicrobial agent. In DPPH scavenging assay IC lessThan sub greaterThan 50 lessThan /sub greaterThan value of the three extracts was found 49.24μg/ml ( lessThan i greaterThan in vitro lessThan /i greaterThan ), 41.66μg/ml ( lessThan i greaterThan in vivo lessThan /i greaterThan ) and 36.14μg/ml (market sample) while the IC lessThan sub greaterThan 50 lessThan /sub greaterThan value of the reference standard ascorbic acid was 53.89 μg/ml. Results suggest lessThan i greaterThan in vitro lessThan /i greaterThan developed plant posses highest antimicrobial and antioxidant activity and thus developed lessThan i greaterThan in vitro lessThan /i greaterThan cultivation technology would provide quality plant material for medicine. Bharat Gami And I. L. Kothari Phyllunthus niruri, axillary bud proliferation, DPPH scavenging, antioxidant activity 78-89