10.22376/ijpbs.2019.10.1.p1-12 Volume 2 Issue 2 2011 (April - June) lessThan i greaterThan Withania somnifera lessThan /i greaterThan , commonly known as Ashwagandha, is a valued herb in Ayurvedic medicine. Roots, leaves and preparations of the plant are traditionally used as tonic, hypnotic, sedative and diuretic. lessThan i greaterThan W.somnifera lessThan /i greaterThan mainly contains withanolides which are specific to the Solanaceae family. Withanolides are biologically active secondary metabolites present in roots and leaves of lessThan i greaterThan W.somnifera lessThan /i greaterThan . In the present study, we have standardized the protocol for the extraction and purification of Withanolide A from the dried lessThan i greaterThan in vivo lessThan /i greaterThan root of lessThan i greaterThan W.somnifera lessThan /i greaterThan . Withanolide A is having a high medicinal value and possesses potent anti-tumor and antioxidant properties. This study involves a detailed investigation about the accumulation of secondary metabolite content in the lessThan i greaterThan in vivo lessThan /i greaterThan root both quantitatively and qualitatively extracts the pure compound using the simple techniques viz., Thin Layer Chromatography, Column Chromatography and High Pressure Liquid Chromatography.  S. Sumithradevi, D. Pradeepa And Kalaiselvi Senthil Withania somnifera roots, Simple Techniques, Pure compound, Withanolide A, High Pressure Liquid Chromatography. 231-236