10.22376/ijpbs.2019.10.1.p1-12 Volume 13 Issue 4 October - December lessThan i greaterThan Mucuna gigantea lessThan /i greaterThan (Willd.) DC. (Fabaceae) is an underutilized endangered medicinal legume distributed in tropical Asia, Japan, Australia, the Pacific islands and Africa. Almost all parts of the plant have been found to contain L-3,4-dihydroxy phenylalanine (L-DOPA), a non-protein amino acid that works as a precursor for the neurotransmitter dopamine. Keeping in view, efforts have been made to develop an efficient, quick and large scale propagation protocol using mature nodal segments of lessThan i greaterThan M. gigantea lessThan /i greaterThan . The aim of the research was to create a quick lessThan i greaterThan in vitro lessThan /i greaterThan regeneration of shoot and root optimisation of the cytokinin and auxin are required. Several cytokinins, 6-benzyladenine (BA), kinetin (Kin), meta-Topolin (mT), Thidiazorone (TDZ) and auxins, Indole-3-butyric acids (IBA), α-Napthalene acetic acids (NAA) singularly or in combinations were supplemented for lessThan i greaterThan in vitro lessThan /i greaterThan shoot multiplication. Among them, BA was efficient in producing several shoots at an ideal dosage of 2.0 mg/l and IBA 0.5 mg/l for roots. The effectiveness of shoot regeneration was also controlled by the strength and NaCl concentration in the basal media. When the NaCl concentration was lowered, the frequency of shoot regeneration tends to increase up to a certain limit. On MS medium supplemented with 2.0 mg/l BA and 0.5 mg/l NAA, the highest response lessThan b greaterThan 83.3% lessThan /b greaterThan with peak number of multiple shoots lessThan b greaterThan c.a. 9.1 lessThan /b greaterThan and maximum average shoot length lessThan b greaterThan 5.7 lessThan /b greaterThan cm were standardised at four sub-cultures. For up-scaling of shoots, lessThan i greaterThan in vitro lessThan /i greaterThan nodal segments were harvested, cultured and highest shoot multiplication was observed on MS+1.0 mg/l BA. lessThan i greaterThan In vitro lessThan /i greaterThan shoots removed from proliferating shoot cultures were best encouraged to root on ½ MS medium supplemented with an ideal concentration of 0.5 mg/l IBA with an average 3.1 root numbers with an average 68% survival rate, the lessThan i greaterThan in vitro lessThan /i greaterThan -raised plantlets with well-developed shoots and roots were successfully established in earthen pots containing garden soil and nurtured in a greenhouse. The finding of this study is the first to show lessThan i greaterThan in vitro lessThan /i greaterThan regeneration of lessThan i greaterThan Mucuna gigantea lessThan /i greaterThan . This plant regeneration procedure, would be valuable for lessThan i greaterThan ex situ lessThan /i greaterThan conservation, reintroduction in wild habitat and commercial application in the pharmaceutical industries, which will change the underutilized to utilized legume. Sanjay K. Madkami, Arpita Moharana, Ramesh C. Mishra and Durga P. Barik Fabaceae; Mucuna gigantea; Mature nodal Explants; Plant Regeneration; Tissue Culture. 1-8