10.22376/ijpbs.2019.10.1.p1-12 Volume 12 issue 4 October - December The goal of this study is to provide current information on the various secondary metabolite classes as well as experimental evidence of antibacterial activity against a wide range of pathogens. lessThan i greaterThan Tinospora cordifolia lessThan /i greaterThan leaves belong to the family Menispermaceae and identified as heart leaves Moonseed. lessThan i greaterThan Invitro lessThan /i greaterThan antimicrobial activity against lessThan i greaterThan Bacillus subtilis, Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Candida albicans lessThan /i greaterThan and lessThan i greaterThan Aspergillus niger lessThan /i greaterThan was studied by Agar well diffusion method with various concentrations. GC- MS analysis was performed to identify major bioactive compounds present in the extracts. The GC – MS analyses revealed the presence of 25 compounds in the ethanol extract and 25 compounds in the leaf extract composition. The antimicrobial analysis revealed that the extract had a strong inhibitory activity against lessThan i greaterThan C.albicans lessThan /i greaterThan among the pathogens tested, with the highest zone of inhibition being 30 mm at a concentration of 100 mg/ml. Our findings indicate that lessThan i greaterThan Tinospora cordifolia lessThan /i greaterThan leaf extract can be used in traditional medicines to treat a variety of diseases D. Muthuselvam and Kaniga. K Ethanol extract, Tinospora cordifolia, Antibacterial and antifungal, GC-MS analysis. 13-20