10.22376/ijpbs.2019.10.1.p1-12 Volume 12 issue 4 October - December Our study aim is to characterize and assess the antimicrobial effect of silver nanoparticles (AgNPs) synthesized from the fruit epicarp of lessThan i greaterThan Glycosmis pentaphylla lessThan /i greaterThan against few crops and human pathogens. Our study suggests a novel method for biosynthesis of silver nanoparticles from the epicarp of lessThan i greaterThan Glycosmis pentaphylla. lessThan /i greaterThan The study confirms the ability of the fruit epicarp extract of lessThan i greaterThan Glycosmis pentaphylla lessThan /i greaterThan for the biosynthesis of silver nanoparticles grown under lessThan i greaterThan in-vitro lessThan /i greaterThan conditions. The green synthesis of AgNPs from (Ethyl alcohol) EtOH extracts of lessThan i greaterThan Glycosmis lessThan /i greaterThan lessThan i greaterThan pentaphylla lessThan /i greaterThan was performed through standard protocols. The synthesized AgNPs were confirmed by colour changes (green to brown) within lessThan 10 minutes and characterized by UV-visible spectral, SEM and TGA analysis (Scanning electron microscope, Thermal gravimetric analysis). Antimicrobial activities of the silver nanoparticles were performed by agar well diffusion method against crops pathogenic fungus and human pathogenic bacteria. The highest antifungal activities of silver nanoparticles were found against  lessThan i greaterThan Colletotrichum lindemuthianum lessThan /i greaterThan and lessThan i greaterThan Alternaria alternata. lessThan /i greaterThan The antibacterial activity was measured through the zone of inhibition against lessThan i greaterThan B. subtilis lessThan /i greaterThan (18 mm), lessThan i greaterThan S. typhimurium lessThan /i greaterThan (17.33 mm), lessThan i greaterThan S. mutans lessThan /i greaterThan  (17 mm) and  lessThan i greaterThan E. coli lessThan /i greaterThan  (17 mm). The antimicrobial potential of AgNPs was determined by minimum inhibitory concentration (MIC), minimum fungicidal concentration (MFC) and minimum bactericidal concentration (MBC) tested against human and plant pathogens. In addition, AgNPs displayed the signi?cant synergistic antibacterial effect when it combined with Streptomycin and Ciprofloxacin in the ratio of 1:1. This eco-friendly, biocompatible and sustainable phytofabrication approach of bioactive AgNP synthesis is a progressive step towards various applications to control few crops (Chilli, and Tomato) and human pathogens in near future. Swapan Kumar Chowdhury, Nayan roy and Indrani mukherjee Swapan Kumar Chowdhury, Nayan roy and Indrani mukherjee 35-46