10.22376/ijpbs.2019.10.1.p1-12 Volume 12 Issue 1 2021 (January-March) lessThan i greaterThan Filicium decipiens lessThan /i greaterThan (family: sapindaceae) is an ornamental plant that grows in Asia and Africa. It contains valuable therapeutic phytochemicals. In India, it is traditionally practiced for diabetic treatment. The present study aimed to determine phytochemical, HPLC profile, lessThan i greaterThan in vitro lessThan /i greaterThan anti-inflammatory, antimicrobial and cytotoxic effect against cancer cell lines using methanolic extract (18.7-600 µg/ml) of lessThan i greaterThan Filicium decipiens lessThan /i greaterThan leaves. The extract was prepared using soxhlet extraction and subjected to preliminary phytochemical screening. It showed the presence of alkaloids, tannins, flavonoids and steroids. Vanillic acid, a marker compound, was used for extract standardization using HPLC. Anti-inflammatory studies were assessed using Murine macrophage 264.7 cells. The extract reduced stimulated NO production at the highest tested concentration (IC lessThan sub greaterThan 50 lessThan /sub greaterThan = 295±2.5µg/ml) while antimicrobial effect was evaluated using broth dilution method. Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) was assessed against lessThan i greaterThan Staphylococcus aureus lessThan /i greaterThan , lessThan i greaterThan Staphylococcus mutans lessThan /i greaterThan , lessThan i greaterThan Enterococcus faecalis lessThan /i greaterThan , lessThan i greaterThan Escherichia coli lessThan /i greaterThan , lessThan i greaterThan Klebsiella pneumonia, Pseudomonas aeruginosa lessThan /i greaterThan , and lessThan i greaterThan Candida albicans lessThan /i greaterThan . The extract was found to be active against Gram-positive and negative microorganisms as well as fungal strain was also observed active. MTT assay was used to evaluate the reduction in cell viability of MCF-7 (Breast adenocarcinoma) and A-549 (lungs carcinoma) cells. The cell viability was displayed in a dose dependent manner and IC lessThan sub greaterThan 50 lessThan /sub greaterThan value was 156.7±1.2 and 217.1±2.3µg/ml for MCF-7 and A-549 cells respectively. This study showed lessThan i greaterThan Filicium decipiens lessThan /i greaterThan methanolic leaves extract exhibited potential anti-inflammatory, antimicrobial and cytotoxic effects. However, further investigations are warranted to establish the mechanistic role of the extract against said activities.  Atiyaparveen I Basarikatti, Sanjay Mishra, Vijayakumar Uppar and Basavaraj Padmashali Filicium decipiens, anti-inflammatory, antimicrobial, HPLC, MTT assay. 73-80