10.22376/ijpbs.2019.10.1.p1-12 Volume 11 Issue 3 2020 (July-September) The increasing prevalence of commercial drug-resistant microbes is a worrisome issue that calls for the search for new antibiotics by isolating microbes that produces active secondary metabolites from diverse environments to obtain new strains and products. In the present study, 25 samples were obtained from marine and marshes sediments in Basrah city, Iraq. The screening revealed that 13 isolates possess antimicrobial activity. Three of these strains (W2, W5, and W11) were found to produce antibacterial and antifungal metabolites, W5 has maximum inhibition zone (28 and 22 mm) was selected against lessThan i greaterThan E. coli lessThan /i greaterThan and lessThan i greaterThan Staphylococcus aureus lessThan /i greaterThan respectively, W2 recorded 14 mm of inhibition zone against lessThan i greaterThan Aspergillus niger lessThan /i greaterThan , while W11 has 13 mm of inhibition zone against lessThan i greaterThan Candida albicans lessThan /i greaterThan . These isolates were identi?ed as lessThan i greaterThan Streptomyces lessThan /i greaterThan sp. TRM46619 (W2), lessThan i greaterThan Micromonospora auratinigra lessThan /i greaterThan SB29 (W5) and lessThan i greaterThan Streptomyces carpaticus lessThan /i greaterThan PES-A23 (W11) depended on amplified 16S rDNA gene by using universal primers. All strains have broad antimicrobial activity against pathogenic bacteria gram-positive and gram-negative, as well as against fungi, and this indicates a promising potential for new antibiotics. Walaa Isaa Ghadhban and Ahmed Abd Burghal Antimicrobial activity, Streptomyces, Micromonospora, Sediment 92-98