10.22376/ijpbs.2019.10.1.p1-12 Volume 11 Issue 3 2020 (July-September) Diabetes mellitus is a group of heterogeneous disorders which arises due to glucose intolerance associated with disagreements in series of metabolism like carbohydrate, protein and lipids. These metabolic alterations emanate from diminished insulin secretion or action and sometimes both. The aim of the present study is to investigate the lessThan i greaterThan in-vitro lessThan /i greaterThan antidiabetic potential of lessThan i greaterThan Luffa acutangula lessThan /i greaterThan leaves of which the study done mainly on the inhibitory potential of carbohydrate hydrolysing enzyme α-amylase and α-glucosidase. The key strategy behind antidiabetic medication is inhibition of these 2 enzymes. There is a significant increase in inhibitory potential of enzyme α-amylase and α-glucosidase in a dose dependent manner with IC lessThan sub greaterThan 50 lessThan /sub greaterThan value 244.5 µg/ml and 673.4 µg/ml with that of standards metformin and acarbose respectively. This result evidenced the potent antidiabetic activity of lessThan i greaterThan Luffa acutangula lessThan /i greaterThan leaves. Thus, it could be concluded that the presence of rich phenolic compounds might be responsible for the management of diabetes. Hence further quantification of specific phytoconstituents responsible for inhibitory activity and its efficacy in animals and humans should be done through lessThan i greaterThan in-vivo lessThan /i greaterThan studies for clinical applications. Shanmuga Priya R* and Ragavan B Luffa acutangula, ?-amylase, ?-glucosidase, diabetes mellitus, antidiabetic medication 86-91