10.22376/ijpbs.2019.10.1.p1-12 Volume 11 Issue 3 2020 (July-September) The plant Acacia leucophloea is reported to have great medicinal value in Indian medicine. The present study deals with the pharmacognostical investigation on leaves of Acacia Leucophloea.Theplant leaves were dried and extracted in Methanol by using soxhlet apparatus.GC-MS chromatogram analysis of the Methanolic extract of Acacia Leucophloea showed 40 peaks indicating the presence of various photochemical constituents. Antimicrobial activity ofmethanolicextracts of Accacia leucophloea leaves were determined by Agar disc diffusion assay against test strains of E. coli, S. aureus, P. acnes, S.typhi.Antioxidant present in plant werequantified by DPPH. Methanolicextract of Acacia Leucophloea showed minimum efficient inhibition of bacterial growth against all gram positive and gram negative bacterial strain due to the presence of lower concentration bioactive phyto constituents like 3-Heptadecanol, Salicin, (E)-Phytol and Hexadecanoicacidmethylester.  Anil F.Bobade Phytochemicalanalysis,GC-MS,Chemical,Composition,Antioxidant, Antimicrobial activity. 1-9