10.22376/ijpbs.2019.10.1.p1-12 Volume 11 Issue 2 2020 (April-June) Chlorpyrifos (CPF) is an organophosphorus pesticide, commonly used in domestic and agricultural areas because of its wide spectra pesticide effect. This study was made to study the effect of CPF on lessThan i greaterThan Channa punctatus lessThan /i greaterThan by measuring gonad tissue's glycogen, protein, total lipid and lipid profile. Adult fish were exposed to different concentrations of Hilban lessThan sup greaterThan (R) lessThan /sup greaterThan (20% EC CPF) for 24 h to get the LC lessThan sub greaterThan 50 lessThan /sub greaterThan . The sublethal concentration (1/3 lessThan sup greaterThan rd lessThan /sup greaterThan and 1/10 lessThan sup greaterThan th lessThan /sup greaterThan of LC lessThan sub greaterThan 50 lessThan /sub greaterThan for three days and seven days respectively) tested for duration study. Among biochemical profiles, in testis, a decrease in carbohydrate content was higher followed by protein and total lipid, whereas in the ovary, a decrease in protein content was higher followed by carbohydrate and total lipid. It represents main biochemical component of gonad that severly damaged by CPF and trigger related hazardous effects. The level of lipid fractions was declined in testis as well as ovary with an increase of CPF concentration. Lipid and lipid fractions are important in hormonal synthesis and normal physiology of gonad. These results support that the organophosphate pesticide not only leads to mortality (immediate effect) but by manipulating gonad biochemical profile it may influence generations as tempering in gamete quality (long-term effect). Abha Mishra and Yogita Devi Chlorpyrifos; Channa punctatus; LC50; Gonad; Biomolecules; Duration response 84-94