10.22376/ijpbs.2019.10.1.p1-12 Volume 10 Issue 3 2019 (July-September) Phylloplane fungi are fungi growing on the surface of the leaf. Leaf and fungi grow in association with each other either symbiotically or asymbiotically. The present investigation explores the presence of potential bioactive compounds present in the leaves of lessThan i greaterThan Garuga pinnata lessThan /i greaterThan and lessThan i greaterThan Colletotrichum gleosporoides lessThan /i greaterThan . The fungus was screened for the presence of bioactive compounds using GC-HRMS. Further these compounds were analysed for anticancer activity against two human cancer cell lines. About 14 bioactive compounds in ethyl acetate of lessThan i greaterThan Garuga pinnata lessThan /i greaterThan leaves & 18 bioactive compounds in the ethyl acetate extracts of lessThan i greaterThan Colletotrichum gleosporoides lessThan /i greaterThan were detected. lessThan i greaterThan Colletotrichum lessThan /i greaterThan showed anticancer activity on cancer cell lines SiHa & SCC – 40 causing 50% inhibition of cell growth of cervical & oral tissues. RAHAT UBAID ANSARI AND SASHIREKHA SURESHKUMAR Garuga; phylloplane fungi; Gas chromatography; anticancer 118-127