10.22376/ijpbs.2019.10.1.p1-12 Volume 10 Issue 3 2019 (July-September) Vulvovaginal candidiasis (VVC) is a disguising infection in an antenatal woman. lessThan i greaterThan Candida lessThan /i greaterThan species lessThan i greaterThan lessThan /i greaterThan are yeast-like fungi present as normal flora in female genitalia and cause infection in individuals with underlying immunocompromised conditions. Several virulence factors are present in lessThan i greaterThan Candida lessThan /i greaterThan species such as production of biofilm, secreted aspartyl proteinase (SAP), phospholipase, lipase, esterase and hemolysin. Biofilm is characterized as organized microbial networks that are connected to a surface and covered in a framework of exo-polymeric material which is responsible for virulence. The aim of this study was to demonstrate the in vitro biofilm forming abilities of lessThan i greaterThan Candida lessThan /i greaterThan species isolated from antenatal women with vulvovaginal candidiasis. lessThan i greaterThan lessThan /i greaterThan This was a prospective study carried out in SRM Medical College Hospital & Research Centre, Chennai, India, from March 2017 to December 2018. A total number of 300 vaginal swabs were collected from antenatal women suspected for symptomatic and asymptomatic vulvovaginal candidiasis. Samples were subjected to lessThan i greaterThan Candida lessThan /i greaterThan species identification. Biofilm was detected by microtiter plate method. lessThan i greaterThan lessThan /i greaterThan Totally 120 lessThan i greaterThan Candida lessThan /i greaterThan species were isolated. Out of 120 lessThan i greaterThan Candida lessThan /i greaterThan isolates, lessThan i greaterThan Candida albicans lessThan /i greaterThan were 44 (37%) and lessThan i greaterThan Non-albicans Candida lessThan /i greaterThan (NAC) were 76 (63%). Among NAC isolates lessThan i greaterThan C. krusei lessThan /i greaterThan was the highest biofilm producer (90%), trailed by lessThan i greaterThan C. tropicalis lessThan /i greaterThan (82%), lessThan i greaterThan C. glabrata lessThan /i greaterThan (77%), lessThan i greaterThan C. parapsilosis lessThan /i greaterThan (75%) and lessThan i greaterThan C. albicans lessThan /i greaterThan (68%). lessThan i greaterThan   lessThan /i greaterThan The present data shows that the prevalence of NAC was higher than that of lessThan i greaterThan C. albicans lessThan /i greaterThan . The lessThan i greaterThan Candida lessThan /i greaterThan isolated from symptomatic antenatal women was found to produce more biofilm than that isolated from asymptomatic women. KANISHKA HRISHI DAS, V. MANGAYARKARASI AND MAITRAYEESEN Vulvovaginal candidiasis, symptomatic, asymptomatic, antenatal, biofilm. 36-41