10.22376/ijpbs.2019.10.1.p1-12 Volume 10 Issue 2 2019 (April-June) The study aims to investigate the efficiency of lessThan i greaterThan Ocimum gratissimum lessThan /i greaterThan against pathogenic bacteria which includes lessThan i greaterThan Escherichia coli lessThan /i greaterThan , lessThan i greaterThan Salmonella entericaser Typhi lessThan /i greaterThan and lessThan i greaterThan Staphylococcus haemolytic us lessThan /i greaterThan using standard microbiological procedures lessThan i greaterThan . lessThan /i greaterThan lessThan i greaterThan Ocimum gratissimum lessThan /i greaterThan was harvested and air dried for 6-7 days. The stuff was processed into fine powdery. Benzene, Hexane, Chloroform, Ethyl acetate served as extraction solvent, and once extraction, the obtained plant extract was weighed and preserved underneath anti-microbial condition.. The extracts were poured into the wells at different concentrations of 50mg/ml, 100mg/ml, 200mg/ml and 400mg/ml. After incubation zones of inhibition were carried out by agar cup method was observed. Among five extracts, the zone of inhibition Screened best in ethyl acetate extract. In case of lessThan i greaterThan Salmonella entericaser Typhi: lessThan /i greaterThan (MTCC 8767) the ethyl acetate extract (400 mg/ml) showed maximum zone of inhibition 17.3 ± 0.2 mm while benzene extract (50 mg/ml) showed minimum zone of inhibition 10.8 ± 0.1 mm In case of lessThan i greaterThan Staphylococcus haemolytic us: lessThan /i greaterThan (MTCC 3383) the ethyl acetate extract (400 mg/ml) showed maximum zone of inhibition 18.0 ± 0.1 mm, while benzene extract (50 mg/ml) showed minimum zone of inhibition 11.6 ± 0.2 mm In the case of lessThan i greaterThan Escherichia coli lessThan /i greaterThan :(MTCC 2872) the ethyl acetate extract (400 mg/ml ) showed maximum zone of inhibition 18.7 ± 0.2 mm, while benzene extract (50 mg/ml) showed minimum zone of inhibition11 ± 0.2mm. Hence lessThan i greaterThan Ocimum gratissimum lessThan /i greaterThan can be employed in developing therapeutic drugs against many bacterium. lessThan i greaterThan Many studies have been reported for the presence of alkaloids, tannins, glucosides, B vitamins, water soluble vitamins etc., in Ocimum gratissimum leaves. lessThan /i greaterThan TANMAY GHOSH, MOHAN KUMAR BISWAS AND RISHIN BHATTACHARYYA Ocimum gratissimum; Escherichia coli; Staphylococcus haemolyticus; Phytochemical constituents; Alkaloid; Zone of inhibition 196-203