10.22376/ijpbs.2019.10.1.p1-12 Volume 10 Issue 1 2019 (January - March) This research tries to evaluate and analyse the anticancer potential of lessThan i greaterThan Solanum villosum lessThan /i greaterThan leaf extract against diethylnitrosamine (DEN) induced hepatocellular carcinoma in experimental rats. The biochemical analysis, tumour markers, hematological parameters, intermediary metabolic enzymes, molecular genetic analysis and histopathological studies were carried out in a systematic manner and it was statistically compared between the different experimental groups. Administration of DEN (Diethylnitrosamine) induced hepatocellular carcinoma it is confirmed based on biochemical parameters and histopathological observations. After the administration of EESV (ethanolic extract of lessThan i greaterThan Solanum villosum lessThan /i greaterThan ) supplement has significantly attenuated these alterations, thereby showing potent anticancer activity of lessThan i greaterThan Solanum villosum lessThan /i greaterThan leaf extract. These findings suggest that EESV reduce hepatic cell damage, and protects the metabolic system against DEN-induced hepatocellular carcinogenesis. Dr. R. VENKATESH AND Dr. K. KALAIVANI Diethylnitrosamine (DEN), ethanolic extact of Solanum villosum (EESV), lactate dehydrogenase (LDH), 5’- Nucleotidase (5’- NT), transaminases, Alpha fetoprotein (AFP) and cancer embryonic antigen (CEA). 90-103