10.22376/ijpbs.2019.10.1.p1-12 Volume 4 Issue 2 2013 (April - June) In this investigation, innate fungal species were isolated from soils of Odisha and screened for their hydrolytic potentials. Utmost numbers of fungi were isolated from botanical garden soil (3 × 10 lessThan sup greaterThan 8 lessThan /sup greaterThan CFU/g), Post Graduate Department of Botany, Utkal University, Odisha, India. Among 35 fungal taxa, lessThan i greaterThan Aspergillus niger lessThan /i greaterThan was most predominant mycoflora followed by lessThan i greaterThan Aspergillus terreus lessThan /i greaterThan . All fungal isolates were accredited to nine genera like lessThan i greaterThan Alternaria, lessThan /i greaterThan lessThan i greaterThan Curvularia, Fusarium lessThan /i greaterThan and lessThan i greaterThan Mucor lessThan /i greaterThan represented two taxa, lessThan i greaterThan Penicillium lessThan /i greaterThan by four taxa, lessThan i greaterThan Rhizopus lessThan /i greaterThan by 3 taxa and lessThan i greaterThan Trichoderma lessThan /i greaterThan represented one species. lessThan i greaterThan Aspergillus lessThan /i greaterThan species were dominant groups that represented about 18 taxa. All isolates were screened for hydrolytic competence and most of the isolates biosynthesized one or more hydrolytic enzymes. lessThan i greaterThan Aspergillus lessThan /i greaterThan lessThan i greaterThan terreus lessThan /i greaterThan exhibited splendid diversity in biosynthesis of all investigated hydrolases. Hence, lessThan i greaterThan A lessThan /i greaterThan . lessThan i greaterThan terreus lessThan /i greaterThan could be a prospective contender in biosynthesizing high valued-low cost industrial enzymes from cheapest substrates addressing the welfare of mankind. BIJAY KUMAR SETHI1, PRATIVA KUMARI NANDA2 AND SANTILATA SAHOO1 * Aspergillus, botanical garden, hydrolases, mycoflora, Trichoderma 89-100