10.22376/ijpbs.2019.10.1.p1-12 Volume 9 Issue 1 2018 (January-March) The study was aimed to evaluate the lessThan i greaterThan in vitro lessThan /i greaterThan anti-inflammatory activity of ethanolic extract of whole plant of lessThan i greaterThan Hybanthus enneaspermus. In vitro lessThan /i greaterThan anti-inflammatory activity was estimated by human red blood cell membrane stabilisation (HRBC) method. The whole plant of lessThan i greaterThan Hybanthus enneaspermus lessThan /i greaterThan was air dried and powdered. The powdered crude plant was cold macerated for 3 days with absolute ethanol, with occasional stirring.. The ethanolic extract of lessThan i greaterThan Hybanthus enneaspermus lessThan /i greaterThan was analyzed in various concentrations as follows 50µg/ml, 100µg/ml, 200µg/ml, 400µg/ml and 800µg/ml. Diclofenac was used as standard anti-inflammatory drug. All the concentrations of lessThan i greaterThan Hybanthus enneaspermus lessThan /i greaterThan ethanolic extract showed effective anti-inflammatory activity in a dose-dependent manner. It was found that the ethanolic extract of whole plant of lessThan i greaterThan Hybanthus enneaspermus lessThan /i greaterThan at a concentration of 50µg/ml produced a percentage inhibition of 20.62% and in 100µg/ml it was increased to 29.88%. Likewise at 200µg/ml the percentage inhibition was 36.54%, which further increased to 56.72% at 400µg/ml. At 800µg/ml concentration it showed maximum inhibition of 69.90%. IC lessThan sub greaterThan 50 lessThan /sub greaterThan value of the extract was found to be 428µg/ml. The ethanolic extract of lessThan i greaterThan Hybanthus enneaspermus lessThan /i greaterThan at a concentration of 800 µg/ml showed potent activity on comparing with the standard drug diclofenac sodium. DR.S.UMAMAHESWARI, SINDHUJA KANNAN, K.S.SRIDEVI SANGEETHA Hybanthus enneaspermus, HRBC, ethanolic extract, anti-inflammatory, diclofenac. 62-65