10.22376/ijpbs.2019.10.1.p1-12 Volume 8 Issue 4 2017 (October - December) The present study aimed at lessThan i greaterThan in vitro lessThan /i greaterThan study of the antioxidant and antibacterial activities of methanol, acetone and hexane extracts of lessThan i greaterThan Annona muricata lessThan /i greaterThan stem by cold and hot methods. The antioxidant activity of these extracts were determined by using DPPH (1,1-Diphenyl -2-picryl hydroxyl) radical scavenging method. It was found that the crude hot extract of methanol solvent of lessThan i greaterThan Annona muricata lessThan /i greaterThan stem had significantly highest antioxidant activity as compare to all other crude extracts. All extracts showed lower antioxidant activity than the standard gallic acid. The antibacterial activity of these extracts were determined by using agar well diffusion method. DMSO was used as a solvent to dissolve the extracts, Ampicillin was taken as a positive control. It was found that the bacteria ( lessThan i greaterThan B.licheniformis lessThan /i greaterThan and lessThan i greaterThan E.coli) lessThan /i greaterThan were largely inhibited by methanol hot extract. The present study thus suggested that the use of this medicinal plant may be exploited for health supplements. SAMREEN BANO and POONAM PRAKASH DPPH (1,1-Diphenyl -2-picryl hydroxyl) radical scavenging, Gallic acid, agar well diffusion, DMSO (dimethyl sulphoxide), Ampicillin, Annona muricata. 178-182