10.22376/ijpbs.2019.10.1.p1-12 Volume 8 Issue 4 2017 (October - December) Antimicrobial activity of seed extract and callus biomass extract of lessThan i greaterThan Linum usitatissimum lessThan /i greaterThan L. was studied using ethanol as solvent against some strains of clinically important pathogens that include bacterial strains lessThan i greaterThan Bacillus cereus lessThan /i greaterThan , lessThan i greaterThan Klebsiella pneumoniae lessThan /i greaterThan lessThan i greaterThan , lessThan /i greaterThan and fungal strains lessThan i greaterThan Candida albicans lessThan /i greaterThan and lessThan i greaterThan Aspergillus niger. lessThan /i greaterThan The extraction was done by the Soxhlet method and its inhibitory effect was investigated using agar disc diffusion method. lessThan i greaterThan Bacillus lessThan /i greaterThan lessThan i greaterThan cereus lessThan /i greaterThan showed the highest zone of inhibition 8.3±0.57 mm for seed extract and 13.3±1.15mm for callus biomass extract, against lessThan i greaterThan Klebsiella pneumoniae lessThan /i greaterThan . These results were compared with the reference drug Amikacin which was found extremely resistant at the concentration of 100 mcg/mL for both lessThan i greaterThan Bacillus cereus lessThan /i greaterThan and lessThan i greaterThan Klebsiella pneumoniae lessThan /i greaterThan . In case of the fungal strains, lessThan i greaterThan Candida albicans lessThan /i greaterThan showed the highest zone of inhibition 9.3±0.57mm for seed extract and 14.6±0.57mm for callus biomass extract, against lessThan i greaterThan Aspergillus niger. lessThan /i greaterThan The reference drug Fluconazole was resistant at the concentration of 150 mcg/mL against lessThan i greaterThan Candida albicans lessThan /i greaterThan but non-resistant against lessThan i greaterThan Aspergillus niger lessThan /i greaterThan . However, it is interesting to note that callus ethanolic extracts revealed a higher degree of antimicrobial activity than seed ethanolic extract. VIJAY D. MENDHULKAR AND SWATI PRAKASH SAKHARE Antifungal and Antibacterial activity. Callus extract Linum usitatissimum , Seed extract. 263-268