Oxidized [1-14 c] linoleic acid and hydrogen peroxide tone might promote anti-atherogenic action and prevent metabolic diseases

10.22376/ijpbs.2019.10.1.p1-12

Volume 8 Issue 4 2017 (October - December) Oxidized [1-14 c] linoleic acid and hydrogen peroxide tone might promote anti-atherogenic action and prevent metabolic diseases Oxidized low density lipoprotein (Ox-LDL) and its various forms are responsible for development of proatherogenic action in in vitro cell culture system. Reactive oxygen species (ROS) can produce detrimental effects that include atherosclerosis. However, mild oxidized products could also impose antiatherogenic action. Present study suggests that HepG2 cells treated with [1- lessThan sup greaterThan 14 lessThan /sup greaterThan C] Linoleic acid formed oxidized linoleic acid (13-hydroperoxy-9,11-octadecadienoic acid) [13-HPODE] and hydrogen peroxide [H lessThan sub greaterThan 2 lessThan /sub greaterThan O lessThan sub greaterThan 2 lessThan /sub greaterThan ] lessThan sub greaterThan . lessThan /sub greaterThan In addition to that cells exposed to oxidized [1- lessThan sup greaterThan 14 lessThan /sup greaterThan C] HPODE showed peroxisomal degradation of oxidized [1- lessThan sup greaterThan 14 lessThan /sup greaterThan C] HPODE to H lessThan sub greaterThan 2 lessThan /sub greaterThan O lessThan sub greaterThan 2 lessThan /sub greaterThan which might be a lipoxygenase enzymatic reaction. Azelaic acid which is lipid peroxidation-derived lipophilic dicarboxylic acid is also formed during the reaction. The generation of this compound can induce anti-atherosclerotic effect. Similarly, in absence of HepG2 cells [1- lessThan sup greaterThan 14 lessThan /sup greaterThan C] linoleic acid and [1- lessThan sup greaterThan 14 lessThan /sup greaterThan C] HPODE independently formed least oxidation. Ox-LDL treatment to HepG2 cells at 0-4 h showed increase in H lessThan sub greaterThan 2 lessThan /sub greaterThan O lessThan sub greaterThan 2 lessThan /sub greaterThan generation. lessThan sub greaterThan lessThan /sub greaterThan Ox-LDL alone showed rise in lipid peroxide and H lessThan sub greaterThan 2 lessThan /sub greaterThan O lessThan sub greaterThan 2 lessThan /sub greaterThan content at 1h. A cellular change was also noticeable effect of Ox-LDL and HPODE treatment. PRATIBHA KAMBLE Linoleic acid; Lipoxygenase; 13-hydroperoxy-9,11-octadecadienoic acid. 112-118