10.22376/ijpbs.2019.10.1.p1-12 Volume 8 Issue 4 2017 (October - December) The aim of the study is to obtain good seedling of anti-diabetic plant Loquat ( lessThan i greaterThan Eriobotrya lessThan /i greaterThan lessThan i greaterThan japonica lessThan /i greaterThan Lindl.) through lessThan i greaterThan in vitro lessThan /i greaterThan germination by using growth stimulator of gibberellic acid (GA lessThan sub greaterThan 3 lessThan /sub greaterThan ) and kinetin. The study is carried out using a completely randomized design with two factors, the concentrations of GA lessThan sub greaterThan 3 lessThan /sub greaterThan (0, 250 and 500 mg/L) and the kinetin (0, 4, 8 and 12 mg/L). The results showed that the technique has successfully shortened the germination time of loquat seed and stimulate the growth development of shoots, leaves and roots. The GA lessThan sub greaterThan 3 lessThan /sub greaterThan and kinetin at optimum condition are found very effective to stimulate seed germination and growth of loquat seedlings. Combination of 500 mg/L GA lessThan sub greaterThan 3 lessThan /sub greaterThan and 8 mg/L kinetin, followed by 250 mg/L GA lessThan sub greaterThan 3 lessThan /sub greaterThan and 4 mg/L kinetin was observed to be the best experimental condition to produce good quality seedling. It was also observed that high concentration of 500 mg/L GA lessThan sub greaterThan 3 lessThan /sub greaterThan alone is very effective to improve the growth of loquat root. The study is promising to obtain good seedling of loquat plants. ISNAINI NURWAHYUNI , HENDA NAULI MARPAUNG AND SUCI RAHAYU In Vitro, Loquat (Eriobotrya japonica Lindl.), Germination, Anti-diabetic plant, Seedling 30-39