10.22376/ijpbs.2019.10.1.p1-12 Volume 8 Issue 2 2017 (April - June) Protocol for lessThan i greaterThan in vitro lessThan /i greaterThan clonal propagation through shoot proliferation of nodal explants of a vulnerable ethnomedicinal Cucurbit lessThan i greaterThan , Trichosanthes tricuspidata lessThan /i greaterThan Lour., was developed. Maximum number of shoots (20.16±0.79) and shoot length (4.16±0.79) was obtained on MS medium supplemented with BAP (4.0 mg/L) + TDZ (0.5 mg/L), after four weeks of culture. Microshoots cultured on MS medium supplemented with IBA (1.5 mg/L) produced adventitious roots. Cytological examination of root tips of plantlets (R lessThan sub greaterThan 1 lessThan /sub greaterThan ) obtained in this study did not reveal mitotic anomalies and the R lessThan sub greaterThan 1 lessThan /sub greaterThan plantlets were morphologically similar to mother plant. About 70% of R lessThan sub greaterThan 1 lessThan /sub greaterThan plantlets transferred to field condition survived and the vine grew well. These results demonstrated that lessThan i greaterThan in vitro lessThan /i greaterThan clonal propagation of lessThan i greaterThan T. tricuspidata lessThan /i greaterThan is technically viable. Rajender. G, D. Bheemanna, P. Sreenu, B. Prasada, K. Rajender and T. Christopher Reuben Trichosanthes tricuspidata Lour., Clonal Propagation, TDZ, Aceto-Orcein 205-209