10.22376/ijpbs.2019.10.1.p1-12 Volume 8 Issue 1 2017 (January - March) α-galactosidase purified from lessThan i greaterThan Acinetobacter lessThan /i greaterThan sp. was immobilized in four different matrices- Calcium alginate, Gelatin, Agarose and lessThan i greaterThan k lessThan /i greaterThan -Carrageenan. On comparative analysis, lessThan i greaterThan k lessThan /i greaterThan -Carrageenan immobilized enzyme showed highest immobilization yield of 68% and storage stability for 30 days. Gelatin showed an immobilization yield of 57%. Soluble enzyme led to decrease of 84% in raffinose and 76% in stachyose concentration in cowpeas whereas lessThan i greaterThan k lessThan /i greaterThan -Carrageenan immobilized enzyme led to decrease of 72% of raffinose and 54% of stachyose thereby decreasing the flatulence to greater extent. SIRISHA E, RANJIT P, LAKSHMI NARASU M k-carrageenan, immobilization, galacto-oligosaccharides, α-galactosidase, cowpeas, flatulence 596-602