10.22376/ijpbs.2019.10.1.p1-12 Volume 8 Issue 1 2017 (January - March) Triton X-100 and Dimethyl sulfoxide (DMSO) were used as permeabilizing agent for the release of campesterol and flavonoids in cell suspension culture of lessThan i greaterThan Blumea lacera lessThan /i greaterThan . The permeabilizing treatment with both the agents (Triton X-100 and DMSO) in lessThan i greaterThan Blumea lacera lessThan /i greaterThan cell culture revealed interesting results indicating appropriate selection and administration of treatment protocol i.e. concentrations and treatment durations. Triton X-100 (1%) for 4 hr treatment duration favored high release of flavonoids (0.096 mg/g) (3.0 fold) as compared to control (0.032 mg/g) in suspension cultures of lessThan i greaterThan Blumea lacera. lessThan /i greaterThan For Campesterol, 0.1% Triton X-100 treatment for 4 hr duration showed marked enhancement (2.092 mg/g, 6.0 fold) over respective control (0.346 mg/g). DMSO treatment with 0.4% concentration and 6 hr duration induced 2.5 fold release in both flavonoids (0.025 mg/g) and Campesterol (0.201 mg/g) in comparison with their respective control. PRIYANKA PATADE, VIJAY D. MENDHULKAR* AND MOINUDDIN VAKIL Blumea lacera, Campesterol, DMSO, Flavonoids, Triton X-100. 476-483