10.22376/ijpbs.2019.10.1.p1-12 Volume 8 Issue 1 2017 (January - March) The study deals with the antimicrobial activity lessThan i greaterThan Entoloma speculum lessThan /i greaterThan , a saprophytic, white fleshy fungi. The pileus varies from 5-10 cm in diameter, stalk being 2-10 cm long, gills are light pink. The antimicrobial activity of different solvent extracts of lessThan i greaterThan E speculum lessThan /i greaterThan against lessThan i greaterThan Xanthomonas campestris lessThan /i greaterThan , lessThan i greaterThan Agrobacterium tumefaciens lessThan /i greaterThan , lessThan i greaterThan Klebsiella pneumonia, Escherihia coli lessThan /i greaterThan , lessThan i greaterThan Candida albicans, Penicillium chrysogenum, Asperillus flavous lessThan /i greaterThan and lessThan i greaterThan Aspergillus niger lessThan /i greaterThan was tested in three concentration 100%, 50% and 25%. The methanol extract showed maximum activity in all the concentration, followed by chloroform. The petroleum ether showed least inhibition in 50% (9mm) and 25% (7mm). lessThan i greaterThan Pseudomonas aeruginosa lessThan /i greaterThan (37mm), lessThan i greaterThan Klebsiella pneumonia lessThan /i greaterThan (34mm) and lessThan i greaterThan Staphylococcus aureus lessThan /i greaterThan (15mm) were maximum inhibited pathogens by methanol chloroform and petroleum ether extracts respectively. The lessThan i greaterThan E. speculum lessThan /i greaterThan inhibited bacteria greater than fungi. The GCMS analysis of methanol extract showed the presence of 1, 2-Benzenediol, which may be responsible for activity of methanol extract against pathogens. JAYASHREE K.K. AND KRISHNAPPA M Entoloma speculum, antibacterial, human pathogens, Klebsiella pneumonia, Gas Chromatography, Mass Spectrometry 289-293