10.22376/ijpbs.2019.10.1.p1-12 Volume 8 Issue 1 2017 (January - March) An encapsulation protocol to obtain synthetic seed was standardized in lessThan i greaterThan Rhinacanthus nasutus. I lessThan /i greaterThan lessThan i greaterThan n vitro lessThan /i greaterThan grown shoot tips and nodal segments were encapsulated at different concentration of sodium alginate (2-5 % w/v) and calcium chloride (25 to 200 mM) was used as a gelling agent. Synthetic seeds were stored at 4 °C for a period of 6 months and re-growth frequency were assessed at different strength of MS medium (Full strength, half strength and quarter strength).The maximum response of lessThan i greaterThan in vitro lessThan /i greaterThan grown encapsulated shoot tips was found to be the highest percentage of multiple shoot re-growth, when compared to nodal segments in the case of production of multiple shoot re-growth. Maximum shooting re-growth response (95%) of encapsulated shoot tips produced multiple shoots (6.0 ± 1.8) re-growth was observed on Murashige and Skoog (MS) medium supplemented with 6- Benzylaminopurine BAP (2.0 mg/l) and nodal segments re-growth response (85%) was observed with of multiple shoots (3.5 ± 0.5) cultured on MS medium with BAP (2.0 mg/l). The healthy shoots retrieved from the encapsulated shoot tips and nodal segments were rooted on half-strength MS medium augmented with Indole-3-butyric acid IBA (0.5 mg/l). R. ELANGOMATHAVAN, S. HARIHARAN, P. KALAIVANAN AND S. NANCY BEAULAH Synthetic seeds, Shoot tips, Nodal segments, Cold storage and Shoot re-growth. 98-105