10.22376/ijpbs.2019.10.1.p1-12 Volume 7 Issue 2 2016 (April - June) Green tea ( lessThan i greaterThan Camellia sinensis lessThan /i greaterThan ) consists of various bioactive components which is useful in new kind of formulation to treat the human diseases. Micro-organisms became resistant to varieties of antibiotics so natural herbal remedy is needed to combat the microbial infections. In the present study, polyphenols such as MQK (myricetin, quercetin, kaempferol) and EGCG (epigallocatechingallate) and C (catechin) were extracted from green tea ( lessThan i greaterThan Camellia sinensis lessThan /i greaterThan ) leaves and tested against test bacteria and fungi. Different concentrations of these polyphenols were examined for antibacterial activities by disc diffusion method. Antimicrobial assay showed significant zone of inhibition against lessThan i greaterThan S. aureus lessThan /i greaterThan (Gram positive bacteria), lessThan i greaterThan P. vulgaris lessThan /i greaterThan (Gram negative bacteria), lessThan i greaterThan Cryptococcus sp lessThan /i greaterThan .(fungus) and lessThan i greaterThan Penicilium sp. lessThan /i greaterThan (fungus). Catechin showed broad spectrum antibacterial and antifungal activities. Minimum inhibitory concentration (MIC) of catechin was 3.12 mgdl lessThan sup greaterThan -1 lessThan /sup greaterThan against lessThan i greaterThan S. aureus lessThan /i greaterThan , lessThan i greaterThan P. vulgaris lessThan /i greaterThan and lessThan i greaterThan Cryptococcus sp lessThan /i greaterThan . whereas at 6.25 mgdl lessThan sup greaterThan -1 lessThan /sup greaterThan inhibited lessThan i greaterThan Penicilium sp. lessThan /i greaterThan MQK and EGCG were especially effective against lessThan i greaterThan Cryptococcus sp lessThan /i greaterThan and lessThan i greaterThan Penicilium sp lessThan /i greaterThan .which gave MIC value of 6.25 mgdl lessThan sup greaterThan -1 lessThan /sup greaterThan . EGCG at same concentration was also effective against lessThan i greaterThan P lessThan /i greaterThan lessThan i greaterThan . vulgaris. lessThan /i greaterThan This study suggests the therapeutic use of green tea against bacterial as well as fungal infections. ARNAB DEB,RAJESH P JAYASWAL,AND PRANAY PUNJ PANKAJ Green tea, Epigallocatechingallate, Catechin, Antibacterial activity, Antifungal activity, Disc diffusion method. 744-748