International Journal of Pharma and Bio Sciences
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10.22376/ijpbs.2019.10.1.p1-12
Volume 7 Issue 2
2016 (April - June)
ANTIMICROBIAL ACTIVITIES OF ENDOPHYTIC BACTERIA ISOLATED FROM COTYLELOBIUM MELANOXYLON (HOOK.F.) PIERRE.
The current study used the culturable endophytic bacterium lessThan i greaterThan Nocardiopsis lessThan /i greaterThan sp. BER-17 isolated from the barks of lessThan i greaterThan Cotylelobium lessThan /i greaterThan lessThan i greaterThan melanoxylon lessThan /i greaterThan to synthesize a novel antimicrobial compounds. lessThan i greaterThan Cotylelobium lessThan /i greaterThan lessThan i greaterThan melanoxylon lessThan /i greaterThan is one of therapeutic plant species used in traditional medicine. It is containing compounds with antibacterial activity and bacterial endophytes that provide bioactive metabolites for medicinal purposes, and sources of novel compounds. An attempt has been made to employ endophytic bacterium isolate from medicinal plants in the synthesis of antibacterial compounds. The objectives of this study was to investigate the antibacterial activities produced by lessThan i greaterThan Nocardiopsis lessThan /i greaterThan sp. BER-17. Dichloromethane endophytic bacterial extract and culture broth were tested for antimicrobial activity using well diffusion method against pathogenic bacteria of lessThan i greaterThan Escherichia coli lessThan /i greaterThan ATCC 35218, lessThan i greaterThan Staphylococcus aureus lessThan /i greaterThan ATCC 25923, lessThan i greaterThan Bacillus subtilis lessThan /i greaterThan FNCC 0061 and lessThan i greaterThan Pseudomonas aeruginosa lessThan /i greaterThan FNCC 0063. The antibacterial activities were assessed based by the presence of clear zones as inhibition zones. Composition of secondary metabolites in the dichloromethane bacterial extract was spectrometrically analyzed using GC-MS. The endophytic bacterial extract activity produced with clear zones against both Gram negative and Gram positive bacterial pathogens. Based on GC-MS result, dichloromethane extract endophytic contain components antibacterial activity.
IDRAMSA, ENDANG SUTARININGSIH SOETARTO, LAURENTIUS. H. NUGROHOAND RARASTOETI PRATIWI
Cotylelobium melanoxylon, culturable endophytic bacterium, Nocardiopsis sp., antibacterial activities.
666-672