International Journal of Pharma and Bio Sciences
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editorijpbs@rediffmail.com (or) editorofijpbs@yahoo.com (or) prasmol@rediffmail.com
10.22376/ijpbs.2019.10.1.p1-12
Volume 7 Issue 2
2016 (April - June)
COMPARATIVE STUDIES ON SEQUENCE, STRUCTURE AND SNPS OF E1 ENVELOPE GLYCOPROTEIN OF CHIKUNGUNYA CIRCULATING IN MAHARASHTRA AND WEST BENGAL STATES OF INDIA
Chikungunya virus (CHIKV) is a female lessThan i greaterThan Aedes lessThan /i greaterThan mosquito-borne alphavirus that recently caused large-scale epidemics of arthritic disease in tropics of Asian region. Molecular substitutions in lessThan i greaterThan E1 lessThan /i greaterThan envelope glycoprotein as well as in other structural and non-structural proteins cause variation in genotypes, infectivity and severity in clinical symptoms. GenBank Isolates lessThan i greaterThan AY424803 lessThan /i greaterThan and lessThan i greaterThan KF818473 lessThan /i greaterThan were associated with epidemics/outbreaks occurred in 1965 and 2012, in Maharashtra and West Bengal states of India respectively. Although lessThan i greaterThan E1 lessThan /i greaterThan glycoprotein based phylogenetic analyses established the former as Asian and the latter as ECSA (East-Central-South-African) genotypes, detailed insight into atomic structures and the effect of acquired mutations of these proteins remain to be worked out. In this study we applied lessThan i greaterThan in silico lessThan /i greaterThan approaches on E1 protein to investigate detailed differential analyses of sequence, atomic model structure and acquired substitutions for their effects on stability and lethality. Physicochemical and sequence studies of these proteins show fine tuning of these properties in reference to the template (3N43). Atomic models remarkably show similar structural features as template including domains topologies, secondary structure arrangements and disulfide bonds. Side chain conformations of models and template, are seen to be similar as both form salt-bridges with almost identical specificity. lessThan i greaterThan In silico lessThan /i greaterThan site directed mutagenesis shows, out of 14 and 2 acquired substitutions in lessThan i greaterThan AY424803 lessThan /i greaterThan and lessThan i greaterThan KF818473 lessThan /i greaterThan , only 3 and none are lethal respectively. Our studies provide detailed structural analyses of lessThan i greaterThan E1 lessThan /i greaterThan glycoprotein, new approaches to assess side chain specificity and judgment of lethality of acquired substitutions that have potential applications in interdisciplinary virology.
BUDDHADEV MONDAL, PARTH SARTHI SEN GUPTA, CHITTRAN ROY, RIFAT NAWAZ UL ISLAM, NILADRI HAZRA AND AMAL KUMAR BANDYOPADHYAY
Modeling, CHIKV, Salt Bridge, mutagenesis, substitution and Lethality
406-417