10.22376/ijpbs.2019.10.1.p1-12 Volume 7 Issue 2 2016 (April - June) Crude extracts from leaves of three medicinal plants namely: lessThan i greaterThan Chenopodium album, Moringa oleifera lessThan /i greaterThan and lessThan i greaterThan Terminalia arjuna lessThan /i greaterThan in four buffers: phosphate buffer saline (PBS), potassium phosphate buffer (PPB), sodium acetate buffer (SAB) and sodium phosphate buffer (SPB), were screened for their antimicrobial activity. The quantity of proteins in the extracts was estimated and antimicrobial activity of the extracts determined on the basis of inhibition zone using Agar well Diffusion Assay and Minimal Inhibition Concentration (MIC). The maximum protein (22.96 mg protein/100 ml) was found in the leaf extract of lessThan i greaterThan M.oleifera lessThan /i greaterThan extracted in PBS buffer. Minimum protein was 7.29 mg/100 ml in lessThan i greaterThan T. arjuna in lessThan /i greaterThan the sodium phosphate buffer.All the plants showed significant activity against eight pathogens tested. The PBS buffer extract of lessThan i greaterThan M.oleifera lessThan /i greaterThan showed the maximum inhibition against lessThan i greaterThan E.coli lessThan /i greaterThan (31±0.23mm) and lessThan i greaterThan A. niger lessThan /i greaterThan (30±0.05 mm) while the minimum inhibition was seen against lessThan i greaterThan P. vulagris lessThan /i greaterThan but none for lessThan i greaterThan Fusarium spp. lessThan /i greaterThan MIC values of all extracts tested against five bacterial species ranged from 11 to 188 μg/ml and against the three fungi between 24 to 387μg/ml, respectively. Extracts in phosphate buffer saline and sodium acetate buffer, had higher activity as compared to that in potassium phosphate and sodium phosphate buffers. SHIKHA KHANDELWAL AND SM PAUL KHURANA Chenopodium album, Moringa oleifera and Terminalia arjuna, antimicrobial activity, Buffers, Staphylococcus aureus, Escherichia coli, Bacillus subtilis, Pseudomonas aeruginosa , Proteus vulgaris, Apergillus niger, Tricophyton rubrum and Fusarium spp. 226-233