10.22376/ijpbs.2019.10.1.p1-12 Volume 7 Issue 1 2016 (January - March) lessThan i greaterThan Helicobacter pylori lessThan /i greaterThan is a known agent associated with Peptic ulcer disease(PUD), however the isolation and identification of lessThan i greaterThan H.pylori lessThan /i greaterThan using conventional methodologies are challenging, hence a study was conducted to isolate lessThan i greaterThan Helicobacter pylori lessThan /i greaterThan from biopsy samples of patients with PUD (Peptic ulcer disease) on selective agar media after the preliminary (Campylobacter like organism) CLO testing and genotyping using genus specific 16SrRNA and characterization of virulent factors viz. cagA, vacA and flagella A and nap A which are the predominant among the isolated clinical strains of lessThan i greaterThan Helicobacter pylori. lessThan /i greaterThan The rate of positive  lessThan i greaterThan H. pylori lessThan /i greaterThan in the collected biopsies after the CLO test was 83% after amplification with genus specific 16SrRNA of lessThan i greaterThan Helicobacter pylori lessThan /i greaterThan . The rates of the virulence genes cagA, vacA were 79.4% (62/78) and 25.6% (20/78), respectively. Seven patients were found colonized with both genotypes (cagA + vacA) having a rate of (3%). cagA status in association with vacA genotype has been implicated as a high risk factor for lessThan i greaterThan H. lessThan /i greaterThan lessThan i greaterThan pylori lessThan /i greaterThan related gastric pathologies. flagellaA, and nap A were found to be prevalent in all the positive samples of lessThan i greaterThan H.pylori lessThan /i greaterThan . Fresh biopsy samples which were subjected to culture media containing antibiotics could obtain only few lessThan i greaterThan H.pylori lessThan /i greaterThan isolates. Therefore a genotypic identification of 16sRNA was found to be highly sensitive for the detection of lessThan i greaterThan H.pylori lessThan /i greaterThan than a bed side CLO test which could be positive for other Co-harboring bacterial species. SUGANYA K*, PREMKUMAR A, SEKAR B, SEKAR T AND SUNDRAN B Clinical specimen, Peptic Ulcer Disease (PUD), Virulent genes, Cytotoxin associated gene, Vaculation associated cytotoxin , 638-643