10.22376/ijpbs.2019.10.1.p1-12 Volume 7 Issue 1 2016 (January - March) The study was aimed to evaluate the impact of probiotic bacteria on HEK–293 kidney cell lines and action on pathogenic microbes. The heat killed and live lessThan i greaterThan L.plantarum lessThan /i greaterThan cultures were treated on the HEK 293 cell lines and on pathogenic bacteria. The percentage of viability of the cell line was carried out by using Trypan blue dye exclusion method, by mtt assay it was proved that different concentrations of Heat killed and live strains of lessThan i greaterThan L.plantarum lessThan /i greaterThan were treated on HEK-293 human embryonic kidney cells. The cell viability was not affected by the liver as well as heat killed strains. An antimicrobial assay was carried out to see the action on pathogens. The strain was most effective against gram positive lessThan i greaterThan Staphylococcus aureus lessThan /i greaterThan and least effective against lessThan i greaterThan Shigella flexneri. lessThan /i greaterThan The heat killed strain of lessThan i greaterThan L.plantarum lessThan /i greaterThan is not harming the cell lines and works against pathogens. PRATHIBA SANDEEP AND P. SARAVANA KUMARI HEK 293 cells, Microorganisms, Heat killed L.plantarum, Probiotics, Mtt assay. 583-587