10.22376/ijpbs.2019.10.1.p1-12 Volume 7 Issue 1 2016 (January - March) In the present study, aqueous extract of lessThan i greaterThan Cucurbita maxima lessThan /i greaterThan seeds was evaluated for its antibacterial activity against five different bacterial strains viz. lessThan i greaterThan Escherichia coli lessThan /i greaterThan , lessThan i greaterThan Staphylococcus aureus lessThan /i greaterThan , lessThan i greaterThan Klebsiella pneumoniae lessThan /i greaterThan , lessThan i greaterThan Enterococcous faecalis lessThan /i greaterThan and lessThan i greaterThan Pseudomonas aeruginosa lessThan /i greaterThan . The susceptibility of bacterial strains against the extract was determined using disc diffusion method. All of the bacterial strains were found to be significantly susceptible against the extract except lessThan i greaterThan P. aeruginosa, lessThan /i greaterThan which was least susceptible showing Zone of Inhibition (ZOI) of 4 mm. However, maximum antibacterial activity of lessThan i greaterThan C. maxima lessThan /i greaterThan seed extract was observed against lessThan i greaterThan E. coli lessThan /i greaterThan and lessThan i greaterThan E. faecalis lessThan /i greaterThan with ZOI of 7 mm. Preliminary phytochemical screening revealed the presence of alkaloids, tannins, saponins, proteins, carbohydrates and glycosides in the extract. The data suggests that lessThan i greaterThan C. maxima lessThan /i greaterThan seed extract could be developed as a promising bactericidal agent for curing several bacterial infections. DEVESH KUMAR KUSHAWAHA, SANJUKTA CHATTERJI, MANJULIKA YADAV AND GEETA WATAL Cucurbita maxima seeds, antimicrobial, phytochemical and bactericidal agent 578-582