10.22376/ijpbs.2019.10.1.p1-12 Volume 7 Issue 1 2016 (January - March) A total of 72 actinomycetes strains were isolated from lessThan i greaterThan Capsicum annuum lessThan /i greaterThan L. rhizosphere soils of Warangal, Khammam, Karimnagar and Mahabubnagar from Telangana State, India. Among these 25 showed antifungal activity against lessThan i greaterThan Colletotrichum capsici lessThan /i greaterThan and lessThan i greaterThan Fusarium oxysporum lessThan /i greaterThan . Among these 25 strains, three (OUA17, OUA18 and OUA27) were grown in starch casein broth for 6 days, centrifuged and powdered samples were extracted by rotary evaporator and were screened for antibacterial activity against bacterial pathogens viz., lessThan i greaterThan Escherichia lessThan /i greaterThan lessThan i greaterThan coli, Staphylococcus aureus, Pseudomonas aeruginosa, lessThan /i greaterThan   lessThan i greaterThan Bacillus subtilis lessThan /i greaterThan and lessThan i greaterThan Klebsiella pneumoniae lessThan /i greaterThan . All the three strains showed growth inhibition against these pathogenic bacteria. Among the three strains OUA27 showed highest inhibition against lessThan i greaterThan E. coli lessThan /i greaterThan . These actinomycetes strains were identified by 16S rRNA sequence as lessThan i greaterThan Pseudonocardia lessThan /i greaterThan sp. OUA17TAVKSP, lessThan i greaterThan Streptomyces lessThan /i greaterThan sp. OUA18TAVKSP and lessThan i greaterThan Streptomyces graminearus lessThan /i greaterThan OUA27TAVKSP, deposited in NCBI database and the accession number given were KP013381, KP013382 and KP013383 respectively. The phylogenetic tree was constructed by using phylogeny.fr online phylogeny programs. T. ASHOKVARDHAN AND K. SATYAPRASAD Actinomycetes, antibacterial activity, bacterial pathogens, Pseudonocardia, Streptomyces 397-402